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pUK

規格:

價格:

編號:B220426

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pUK

商品貨號

B220426

Designations

pUK

Depositors

B Huang

培養基

Biosafety Level

Vector Information

Size (kb): 2.4300000667572020

Vector: pUK (plasmid)

Promoters: Promoter tac

Construction: pUC19, pKK223-3

Marker(s):ampR

Construct size (kb): 2.430000066757202

Features: marker(s): ampR
promoter: tac
replicon: pMB1
MCS: HindIII-EcoRI
ribosome-binding site: Shine-Dalgarno
transcription terminator: rrnB

Applications

expression vector

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--2.4; BamHI--2.4; HindIII--2.4.

A small, high-copy-number vector suitable for both in vitro (using the coupled transcription/translation system of Zubay) and in vivo expression.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Huang B, et al. A small, high-copy-number vector suitable for both in vitro and in vivo gene expression. Gene 151: 143-145, 1994. PubMed: 7828863

Shipped

freeze-dried

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